- What it is. Lab Pro's Isopropyl Alcohol 99% HPLC is an HPLC-grade isopropyl alcohol (IPA, isopropanol, 2-propanol; CAS 67-63-0) certified to ACS/USP/NF standards and supplied in 1-gallon fluorinated poly bottles. It is built for chromatography, analytical chemistry, and pharmaceutical research, where solvent quality directly affects results. The gallon format suits labs that run HPLC methods and sample preparation daily and need a dependable, documented gallon of isopropyl alcohol on the shelf. It also suits QC labs that need traceable solvent lots.
- Why HPLC grade matters. Lab Pro's Isopropyl Alcohol 99% HPLC is listed at a 99.90% assay and purified to remove contaminants that could interfere with analysis. In chromatography, impurities in the mobile phase or rinse solvent can show up as ghost peaks, raise baseline noise, or shift retention, so analysts rely on HPLC-certified solvents for reproducible runs. The ACS/USP/NF certification also gives pharmaceutical and analytical labs a recognized standard to cite in methods and quality records.
- Purity specifications. HPLC isopropanol from Lab Pro carries tight limits: water at 0.05% or less, residue after evaporation at 0.001% or less, and color capped at 10 APHA, so the liquid stays clear and colorless. Low water content keeps solvent strength consistent in moisture-sensitive methods, and near-zero residue leaves instrument parts, glassware, and sample vials clean after rinsing. These limits match the needs of sensitive analytical methods and cleaning steps.
- How it is used in the lab. Isopropyl alcohol is miscible with water and most organic solvents, which makes it useful as a mobile-phase component, a strong rinse solvent for columns and flow paths, and a cleaning solvent for sensitive glassware and instruments. It boils at about 181 °F and evaporates quickly, so rinsed parts dry fast without leaving film. Labs also use it for sample preparation, compound purification, and surface preparation in pharmaceutical research. Those uses all depend on the same consistent, low-residue purity.
- Safety profile buyers should know. Isopropyl alcohol is a Class IB flammable liquid: Lab Pro lists a flash point of 53.6 °F, and NIOSH gives explosive limits of 2.0% to 12.7% in air. Its vapor is about twice as heavy as air and can collect at floor level. OSHA sets a permissible exposure limit of 400 ppm as an 8-hour average, and ACGIH recommends 200 ppm, so work with ventilation, gloves, and eye protection, and keep containers closed.
- Packaging and intended use. Lab Pro ships Isopropyl Alcohol 99% HPLC in vacuum-sealed, 1-gallon fluorinated poly bottles (four per case), separated by fiber box dividers for safe transport, with next-day shipping available on the West Coast. It is intended for laboratory, research, and further manufacturing use and may serve as a process solvent or excipient, but it is not an active pharmaceutical ingredient or a medical disinfectant. For low-residue cleanroom cleaning, Lab Pro also stocks Isopropyl Alcohol 99% Cleanroom Grade LP.
Recommended For
- HPLC and chromatography: a mobile-phase component and strong rinse solvent for columns and flow paths
- Sample preparation and purification: dissolves and prepares samples and purifies compounds in research labs
- Instrument and glassware cleaning: residue-free rinsing of sensitive glassware, vials, and instrument parts
- Pharmaceutical research surface prep: prepares surfaces ahead of sterilization and analysis
- Synthesis and analytical chemistry: a high-purity solvent for processes where contaminants would skew results
Product Details
- Chemical Name: Isopropyl Alcohol (2-Propanol)
- Synonyms: IPA, isopropanol, sec-propyl alcohol
- CAS Number: 67-63-0
- Formula: (CH3)2CHOH
- Molecular Weight: 60.1
- Grade: HPLC; ACS/USP/NF
- Assay: 99.90%
- Appearance: Clear, colorless liquid
- Color (APHA): 10 max
- Water: ≤0.05%
- Residue after Evaporation: ≤0.001%
- Density: 0.785 g/mL at 25 °C
- Boiling Point: 181.4 °F
- Freezing Point: -127 °F
- Flash Point: 53.6 °F
- Vapor Pressure: 43.99 hPa at 20 °C (68 °F)
- Vapor Density (air = 1): 2.07
- Explosive Limits: LEL 2.0%; UEL 12.7%
- Solubility in Water: Miscible
- Flammability Class: Class IB flammable liquid
- NFPA Ratings: Health 1 · Fire 3 · Reactivity 0
- DOT ID: UN1219
- Exposure Limits: OSHA PEL 400 ppm TWA · ACGIH TLV 200 ppm TWA, 400 ppm STEL
- Incompatible With: Strong oxidizers, acids, chlorine, acetaldehyde, ethylene oxide, isocyanates
- Container: Vacuum-sealed 1-gallon fluorinated poly bottle; 4 per case with fiber box dividers
FAQs
What is the difference between HPLC grade and cleanroom grade isopropyl alcohol?
HPLC grade IPA is certified for chromatography, while cleanroom grade IPA is made for low-residue cleaning in controlled environments. Lab Pro's HPLC grade also carries ACS/USP/NF certification for analytical and pharmaceutical methods. If the solvent goes into an instrument or method, choose HPLC grade; for wipe-downs and parts cleaning, cleanroom grade fits.
Why do impurities in HPLC solvents cause problems?
Impurities can produce ghost peaks, raise baseline noise, and shift retention times, especially in gradient methods where contaminants concentrate on the column and elute later. That makes results harder to reproduce and validate. Using HPLC-certified solvent and keeping bottles sealed between uses helps keep chromatograms clean and comparable from run to run.
How is isopropyl alcohol used in HPLC?
Isopropyl alcohol is used as a mobile-phase modifier, especially in normal-phase and some reversed-phase methods, and as a strong flushing solvent to clean columns and remove strongly retained compounds. Its relatively high viscosity raises back pressure, so methods using it often run at lower flow rates or elevated temperatures.
Should HPLC solvents be filtered and degassed before use?
Yes, in most methods. Filtering through a fine membrane removes particles that can block frits and columns, and degassing removes dissolved air that causes bubbles, pump instability, and noisy baselines. Many systems include inline degassers, but filtering prepared mobile phases remains standard practice in validated methods. Log preparation dates on every bottle.
Can this HPLC isopropyl alcohol be used as a disinfectant?
No. Lab Pro's Isopropyl Alcohol 99% HPLC is not intended as a medical disinfectant; it is designed for chromatography, pharmaceutical, and analytical applications. For disinfection, Lab Pro's Isopropyl Alcohol 70/30 (USP/NF) is the better fit, because its water content improves microbial kill compared with 99% IPA. Keep the two clearly labeled.
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