- What it is. Lab Pro's Acetone HPLC Grade is a high-purity acetone (2-propanone, dimethyl ketone; CAS 67-64-1) produced for chromatography, analytical testing, and other sensitive laboratory methods. It is supplied in 1-gallon fluorinated poly bottles, a convenient format for labs, research facilities, and pharmaceutical and biotech operations that use acetone HPLC-grade solvent every day and need reliable, consistent quality from bottle to bottle. Lab Pro also references the manufacturer part number 32900HPLCCSGF.
- Why HPLC grade matters. Lab Pro lists this HPLC grade acetone at a minimum 99.5% assay, produced under strict quality controls to minimize contaminants that could interfere with sensitive methods. In chromatography, trace impurities in a solvent can produce ghost peaks or noisy baselines and make results harder to reproduce. Using an HPLC-grade solvent gives analysts a dependable baseline and supports consistent results across runs, instruments, and analysts. It is a practical default for labs that standardize solvent grades.
- Purity specifications. Acetone HPLC Grade from Lab Pro carries tight limits: residue after evaporation at 0.001% or less, water at 0.5% or less, and color capped at 10 APHA, so the liquid stays clear with a characteristic odor. Near-zero residue matters when acetone is the last solvent to touch glassware, sample vials, or instrument parts, because anything left behind could contaminate the next analysis or sample preparation step. These limits are listed on Lab Pro's product page.
- How it is used in the lab. Acetone is fully miscible with water and most organic solvents and dissolves oils, resins, and many organic compounds. It boils at about 133 °F and has a vapor pressure of 245.3 hPa at 25 °C, so it evaporates quickly and leaves surfaces dry within moments. That makes HPLC-grade acetone useful for chromatography, rinsing and drying glassware, degreasing instruments, organic synthesis, and sample preparation in pharmaceutical and biotech work. Rinsed glassware is ready for reuse almost immediately.
- Safety profile buyers should know. Acetone is a Class IB flammable liquid: Lab Pro lists a flash point of -4 °F (-20 °C), and NIOSH gives explosive limits of 2.5% to 12.8% in air. Its vapor is about twice as heavy as air and can travel to distant ignition sources. OSHA sets a permissible exposure limit of 1,000 ppm, and NIOSH recommends 250 ppm as an 8-hour average, so work with ventilation and keep containers closed.
- Packaging and intended use. Lab Pro ships Acetone HPLC Grade as four fluorinated poly gallon bottles per case, in a fiber box with cardboard bottle dividers, and orders ship within 24 hours from its California warehouse. Safety Data Sheets and technical specifications are available. The product is intended for laboratory, industrial, or research use and may serve as a process solvent or excipient, but it is not intended as an active pharmaceutical ingredient. Bottles rotate easily on solvent shelves.
Recommended For
- HPLC and chromatography: a high-purity solvent for separation and analysis methods
- Glassware and instrument cleaning: dissolves residues and dries fast without leaving contaminants
- Organic synthesis and sample prep: a reagent and solvent for reactions and sample preparation
- Pharmaceutical and biotech work: a process solvent in drug development and biopharmaceutical labs
- Industrial and research processes: applications that need high-purity acetone in controlled environments
Product Details
- Chemical Name: Acetone (2-Propanone)
- Synonyms: Propanone, dimethyl ketone, methyl ketone, beta-ketopropane, pyroacetic ether
- CAS Number: 67-64-1
- Linear Formula: CH3COCH3
- Molecular Weight: 58.08
- PubChem Substance ID: 329752967
- Manufacturer Part Number: 32900HPLCCSGF
- Grade: HPLC
- Assay: NLT 99.5%
- Appearance: Clear liquid with characteristic odor
- Color (APHA): 10 max
- Water: ≤0.5%
- Residue after Evaporation: ≤0.001%
- Density: 0.791 g/mL at 25 °C
- Boiling Point: 132.8 °F
- Freezing Point: -140 °F
- Flash Point: -4 °F (-20 °C), closed cup
- Vapor Pressure: 245.3 hPa at 25 °C (77 °F)
- Explosive Limits: LEL 2.5%; UEL 12.8%
- Solubility in Water: Miscible
- Flammability Class: Class IB flammable liquid
- DOT ID: UN1090
- Exposure Limits: OSHA PEL 1,000 ppm TWA · NIOSH REL 250 ppm TWA
- Incompatible With: Oxidizers, acids
- Container: 1-gallon fluorinated poly bottle; 4 per case in fiber box with dividers
FAQs
Is acetone used as an HPLC mobile phase?
Acetone is used less often as a mobile phase than acetonitrile or methanol because it absorbs strongly in the UV range, which interferes with UV detection at common wavelengths. It is widely used for rinsing, cleaning flow paths and glassware, sample preparation, and methods with detectors that tolerate its UV absorbance.
What is the difference between HPLC grade and cleanroom grade acetone?
HPLC grade acetone is made for analytical work where impurities could interfere with results, while cleanroom grade acetone is made for low-residue cleaning in controlled environments. Lab Pro stocks both; choose HPLC grade for analysis and sample prep, and cleanroom grade for wipe-downs and parts cleaning. Using the right grade avoids paying for purity the task does not need.
How long does Acetone HPLC Grade stay usable?
Shelf life depends on storage, and Lab Pro notes it typically extends to several years when the bottle is kept tightly sealed in a cool, dry place away from sunlight. Check the SDS and certificate details, and inspect for changes in appearance or odor before using older stock.
Which containers and tubing are compatible with acetone?
Polyethylene, polypropylene, PTFE, and glass resist acetone well. Acetone dissolves or damages ABS, polystyrene, and many acrylics, and can attack some tubing and seals. In HPLC systems, check that seals, fittings, and tubing are rated for acetone before introducing it into the flow path. When unsure, consult the instrument manufacturer.
Why is acetone stored in fluorinated bottles?
Fluorination treats the inner surface of a polyethylene bottle to improve solvent resistance, reducing permeation and the risk that bottle material leaches into the acetone. That protects purity during storage, which matters for HPLC work where trace contaminants can affect results. Keep bottles capped between uses for the same reason.
Related Products
