HABITAT photo ferment

* may be combined with double-walled vessel packages

The HABITAT photo ferment control unit package of the HABITAT research bioreactor is used to monitor and control the bioprocess. With the help of the LED panels included in the scope of delivery, the system can be used as a photobioreactor, for example for algae cultivation. The adjustment of the light intensity enables the simulation of the day-night rhythm. Furthermore,, the package includes the control unit with all connection options for the gas supply, liquid addition, sensors and temperature control as well as a tablet for simple and clear operation of the bioreactor.

Control and monitoring
The bioprocess and all associated test parameters can be easily controlled and monitored via the tablet attached to the control unit and with the help of the intuitive and easy-to-use software. Depending on the type of cultivation, you can choose between the operating modes batch, fed-batch and perfusion/continuous. The new Chaotic Mixing function ensures faster and more effective mixing when required. The 10.4-inch tablet allows direct access to all actuators directly on the main screen. A clear calibration management, a diagram overview and the complete documentation of the process round off the operation. A software version that meets FDA CFR Part 11 requirements is available as an option.

Gas supply
Built in mass flow controllers for 4 separate gas lines (for N2, O2, air and CO2) ensure precise, individually adjustable gassing, ideally tailored to the needs of your cells. Flow rates of 0.1-20 l/min can be achieved.

Liquid supply
4 integrated Watson Marlow pumps, adjustable in direction and speed, give you the variability to pump in and pump out different liquids (like acid, base, anti-foam agent, feeding solutions).

Sensors
HABITAT cell enables the measurement of the following parameters by means of sensors:
• pH
• DO (dissolved oxygen)
• temperature
• filling level
• foam

Temperature control
The constant and precise temperature control during the cultivation is guaranteed by the heating sleeve, which is adapted to the respective vessel size. (Heating sleeve is included with the HABITAT cell vessel package).

All features at a glance
> compact, space-saving design, dimensions: 223 x 402 x 450 mm (WxDxH)
> large tablet for clear operation (10,4 inch)
> intuitive, easy-to-use software with a lot of functionalities.
> 4 integrated fast-load pumps (Watson Marlow)
> connectivity: USB, PC, RS232, ethernet, external signal input, external pump, single-use unit, thermostat
> data storage
> gas supply with 4 built-in mass flow controllers: e.g. for O2, Luft, N2, CO2
> Status LED display: direct error display by indicator light

Note: Only a control tower package and the separately available vessel package make up a functional unit.
Scope of delivery

• HABITAT photo ferment S099
• Tablet with software for bioreactor
• HA.pl. Peltier cooling element
• LED panel (2pcs)

Technical Data

Controller	Fermenter
Operating mode	timer, continuous and program operation
Display	Tablet PC
Operation	touch screen
Data memory size	32 GB
Auto restart after power failure	yes
Ethernet interface	yes
Single use reactor interface	yes
Thermostat interface	yes
Ext. pump interface	yes
Filter heater	yes
LED light panel	yes
Stirring motor	yes
Calibration guide tepmerature	yes
Calibration guide pH	yes
Calibration guide pO2	yes
pH - temperature compensation	yes
Mode hose filling	yes
Stirrer control mode	off, manual, profile, Cascade-pO2 control, chaotic
Speed range	1 - 2200 rpm
Speed deviation >= 100 rpm	±2 %
Speed deviation <= 100 rpm	±5 rpm
Speed display	TFT
Reversible direction of rotation	yes
Timer	yes
Timer display	TFT
Time setting range	1 - 120 min
Heating blanket	yes
Heat output	250 W
Heat control	TFT
Temperature control	PID
Working temperature	5 - 80 °C
Connection for ext. temperature sensor	PT1000
Temperature display	yes
Temperature measuring range	0 - 100 °C
Temperature measurement resolution	0.1 K
Temperature control accuracy	±0.2 °C
Temperature limit min.	0 °C
Temperature limit max.	110 °C
pH measurement	yes
pH control mode	off, auto, profile
pH value display	TFT
pH measuring range	0 - 14 pH
pH measurement resolution	0.01 pH
Accuracy of pH measurement	0.02 pH
Conncetion for pO2 sensor	yes
pO2 control mode	off, manual, auto, profile
Measurement range pO2 min.	0 %sat
Measurement range pO2 max.	200 %sat
Resolution pO2 measurement	0.01 %sat
Accuracy of pO2 measurement	0.3 %sat
Gas in connection Air	6 mm
Gas out connection Air	6 mm
Gas in connection O2	6 mm
Gas out connection O2	6 mm
Gas in connection N2	6 mm
Gas out connection N2	6 mm
Gas in connection CO2	6 mm
Gas out connection CO2	6 mm
Aeration mode	continuous & automatic
Display flowrate	l/min, cc/min
Input pressure	200 - 3000 mbar
Pressure out min.	200 mbar
Pressure out max.	1000 mbar
Flowrate Air min.	0 cc/min
Flowrate Air max.	20000 cc/min
Flowrate O2 min.	0 cc/min
Flowrate O2 max.	20000 cc/min
Flowrate N2 min.	0 cc/min
Flowrate N2 max.	20000 cc/min
Flowrate CO2 min.	0 cc/min
Flowrate CO2 min.	20000 cc/min
Measuring and control accuracy of the gas control units	2 % fsr
Peristaltic pump	yes
Numbers of pump heads	4
Pump head function	Acid, Base, AntiFoam, Level
Pump head	Watson Marlow 114DV
Number of pump tubes	4
Number of pump rollers	4
Flow rate min.	1.0 ml/min
Flow rate max.	270 ml/min
Pump Speed min.	100 rpm
Pump Speed max.	400 rpm
Pump reversible direction of rotation	yes
Pump speed display	TFT
Inner tube diameter min.	0.5 mm
Inner tube diameter max.	4.8 mm
Tube outer diamter min.	4.1 mm
Tube outer diamter max.	8.4 mm
Tube material	Silicone
Tube hardness	50 - 60 Shore A
Dimensions (W x H x D)	224 x 624 x 405 mm
Weight	14 kg
Permissible ambient temperature	5 - 40 °C
Permissible relative humidity	80% (up to 31°C), decreasing linearly to max. 50 (@40°C)
Protection class according to DIN EN 60529	IP 20
RS 232 interface	yes
USB interface	yes
WiFi Interface	yes
Voltage	100 - 240 V
Frequency	50/60 Hz
Power input	300 W
Power input max.	1100 W
Power input standby	10 W
Fuse	2x T5.0A 250V